What concepts are presented in "Digesting a
Gummy Bear in Trypsin"? Enyzme
types vary along the digestive tract.
The
organ from which Trypsin originates is the pancreas. The
difference between a protein, a peptide and an amino acid.
Trypsin is specific to cut apart proteins after the amino acids lysine and arginine (as long as they are not
followed by a proline)
Enzymes work best
at physiological temperature (37oC) Warm
liquids dissolve a gummy bear faster than cold liquids. Definition
of a hypothesis. The varying pHs across the
digestive tract. The harsh environment of the
digestive system causes cells to renew themselves every few days
Controlling for variables is important in experimentation. The uses of trypsin in research labs: protein sequencing and removing adherent tissue culture cells
from a dish.
One use of trypsin in medicine:
to dissolve blood clots Trypsin is used to pre-digest
pre-packaged food. An example of a disease that
involves trypsin. | Materials:
4 green gummy bears, 20 mls 37oC trypsin (5x), 20mls 4oC trypsin (5x), 20
mls 37oC Phosphate Buffered Saline (1x), 20mls 4oC Phosphate Buffered Saline (1x) Equipment: 4 stir
plates 4 small stir bars
4 50ml erlenmeyer flasks
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